Bioactive zinc-doped sol-gel coating modulates protein adsorption patterns and in vitro cell responses

Zinc is an essential element with important role in stimulating the osteogenesis and mineralization suppressing osteoclast differentiation. In this study, new bioactive ZnCl 2 -doped sol-gel materials were designed to be applied as coatings onto titanium. The biomaterials physicochemically characterized cellular responses evaluated vitro using MC3T3-E1 osteoblasts RAW264.7 macrophages. effect of Zn on adsorption human serum proteins material surface was through nLC-MS/MS. incorporation did not affect crosslinking network. A controlled 2+ release obtained, reaching values below 10 ppm after 21 days. no cytotoxic lead increased gene expression ALP, TGF-?, RUNX2 osteoblasts. macrophages, increase IL-1?, IL-4 accompanied by a reduced TNF-? liberation. Proteomic results showed changes patterns associated immunological, coagulative, regenerative functions, dose-dependent manner. variations protein might downregulation NF-?B pathway, thus explain observed biological effects into biomaterials. Overall, these demonstrated their potential promote bone tissue regeneration. • New Zn-doped developed characterized. could improve regeneration anti-inflammatory effects. correlation between response established. better understanding biomaterials-tissue interactions achieved.